NOTE
This section is valid for the software version 1.0.0 or newer (1.x). If your Kilobaser has an older software version (0.9.x), please contact the Kilobaser support to update your device or provide you with a suitable manual. You can check the software version in the settings/system in the section System information.
1 Settings overview
Settings are found in the dropdown menu.
1.1 Networkadm
WiFi Settings: Disable or enable WiFi and choose the network you want to connect to by pressing “select network”. The supplied WiFi USB dongle needs to be plugged into one of the back USB ports. When WiFi is enabled and connected, the current IP address is shown below the name of the network. If the IP address is not visible after connecting to a network, refresh the page (Dropdown menu → Refresh). If the connection cannot be established, please use the Ethernet cable connection or contact the Kilobaser support.
Ethernet Settings: View network settings of the dynamic generated address if the device is connected with an Ethernet cable (cable not supplied by Kilobaser).
Hostname: If you wish to change the default hostname of your Kilobaser, type the new hostname and save it. You can connect to the Kilobaser remotely via https://YOUR_HOSTNAME.lan, where YOUR_HOSTNAME is your selected hostname.
1.2 Security
When viewed on local touchscreen display:
On the touchscreen display you can allow yourself and your colleagues remote access to Kilobaser's local web interface. Activate device visibility if you want to connect to the device from your PC. Make sure that the device is connected to your network.
Making a remote connection to the Kilobaser requires a TLS certificate and keys. You may provide your own or let the Kilobaser generate one for you. If you want to provide your own certificate and key, plug in a USB drive and press the IMPORT button. You will be prompted to select the key first and secondly the certificate. For each selection you have to press the CONFIRM button. Finalize your selection by pressing the SAVE button.
When viewed remotely via the web interface:
Changing the password of the current user is the only option available remotely.
1.3 System
Demo modeadm distributors: When demo mode is activated, the synthesis process of the Kilobaser is replaced with short procedure protocols to demonstrate basic handling and functionality. Demo mode can be enabled or disabled by pressing the toggle switch. Demo mode can only be activated when the queue is empty and there is no reagent cartridge in the device. If the cartridge is still in the device, the "Manual cleaning" card will be added to the queue. After cleaning, the demo mode can be enabled by pressing the toggle switch.
Emergency Modeem: If the cartridge wasn’t removed after entering the emergency mode, you can again attempt to remove it by pressing EJECT CARTRIDGE. You can leave the emergency mode at your own risk by pressing DISABLE EMERGENCY MODE.
Online accessadm: Allow the Kilobaser support team to access your machine remotely by enabling the online remote access. Make sure that the Kilobaser is connected to a network.
Factory resetadm: Perform a factory reset if you wish to delete all saved user data and synthesis history.
Date/Time Localization: Choose a date/time format and a time zone from the list.
System information: Important information about your Kilobaser. If you need support with your Kilobaser, please give us the information presented here.
1.4 Usersadm
By pressing ADD USER you can create a new user. You can edit the name, password and the role of the user. Admin role offers more options (marked as "adm" in this chapter). Press SAVE to save the user. Note that after saving, user’s login name cannot be changed anymore. If you want to change the login name, delete the user and create a new one. Press the trashcan icon to delete an existing user. A user has to be created if you want to connect to your Kilobaser via a remote web interface.
1.5 Cartridge
Select cartridge type: “Chosen cartridge type” shows the current chosen cartridge type. To change the cartridge type, press the SELECT button, then choose the cartridge type you plan to use from the list and press confirm. The screen returns to the queue, to which all the processes that need to be performed to initialize the cartridge type are automatically added.
Remove consumables: You can initialize the cartridge removal process by pressing START WITHOUT CLEANING or start the automatic cleaning process by pressing START WITH CLEANING. Before you start an automatic cleaning process, make sure to have a cleaning cartridge available. Please read the instructions on the display and those in the respective chapters of this manual before initializing. After pressing confirm, the process card is added to the queue.
Manual Cleaning: Pressing the ADD TO QUEUE button adds the "Manual cleaning" card to the queue. Please read the instructions on the display and those in the respective chapters of this manual before initializing.
adm These sections are available in the remote web interface only to useres with admin privileges. ↩Network, ↩Demo mode, ↩Remote support, ↩Factory reset, ↩Users
distributors This option is only available to licensed distributors. ↩Demo mode
em This option is only available if the device is in the emergency mode. ↩Emergency mode
2 Synthesis preparation
2.1 Cartridge type selection
Depending on the reagent cartridge used, you have to select the appropriate cartridge type in the settings/cartridge. When a new cartridge type is selected, the "Cartridge setup" card is added to the queue.
If the cartridge type is changed when a reagent cartridge is already in the Kilobaser, the "Manual cleaning" card is added to the queue to clean the device before the cartridge setup can be started.
The chosen cartridge type is represented as the color of the area in the up-right corner of the display. It can also be checked by clicking the “Consumables information” button or the "Additional sequence information" button as well as in the settings/cartridge. The color and the “Consumables information” button appear as soon as the first process in the chosen cartridge type is started.
2.2 Chip type selection
While some cartridge types can only be used in combination with standard microfluidic chips, modification cartridges can be combined with either standard chips to synthesize standard oligonucleotides or with modification chips to synthesize labeled oligonucleotides. Click on the “Chip type” button, select the appropriate chip type from the list and press confirm. Now you can enter the desired sequence with the selected chip type. Once a sequence is added to the queue, the label of the chosen chip type is shown in the sequence card. By clicking on this field the chip type can be changed. The chosen chip type can also be checked by clicking the “Consumables information” button or the "Additional sequence information" button. Currently inserted chip is represented as the color of the semi-circle in the up-right corner of the display.
2.3 Queue
A synthesis is prepared by adding a sequence to the queue. After selecting suitable cartridge and chip types, a sequence can be added by several means:
a) Direct input using the touchscreen display: Tip on the base buttons to enter the desired sequence and then click on the ADD SEQUENCE TO QUEUE button.
b) Direct input or copy&paste from your PC using the remote web interface: Click on the base buttons or use the keyboard to enter the sequence. Only the letters A, C, G and T (A, G, C and U with the RNA cartridge) can be entered. Alternatively, paste a copied sequence into the “Enter DNA sequence” field. Only the letters A, C, G and T (A, G, C and U with the RNA cartridge) can be pasted into the field. Then click on the ADD SEQUENCE TO QUEUE button to queue the sequence.
c) Import a sequence from a USB stick: First insert a USB stick with sequence file(s) in FASTA format into one of the USB ports. Then click on the IMPORT button and choose a sequence file. A FASTA file viewer appears with information about the sequence(s) to be imported. After pressing CONFIRM, the sequence(s) is/are directly added to the queue.
Once added to the queue, a sequence card is created. The most important information of the sequence is presented here, such as duration, molar mass (including all possible modifications), the number of nucleotides and the expected concentration at a resuspension volume of 20 µL. By pressing the "Additional sequence information" button you can check the selected chip and cartridge type of the sequence, as well as get information about the resuspension protocol and application notes. You can change the name of the sequence (by default “Oligo YYYY-MM-DD.X”, X being the consecutive number of runs done in the same day), the sequence and the chip type by clicking on the respective field.
Apart from the sequence cards, special operation cards can appear in the queue. Some are automatically added as they are bound to the next process, while the others can be manually added or deleted. Special operations will be discussed in more detail later in this chapter.
Sequence and special operation cards are added in order of creation, however the queue can be rearranged by moving the cards up or down the queue. This is accomplished by clicking the “Move up/down the queue” arrow on the side of a card or by dragging and dropping it to a desired position. A card can be deleted from the queue by selecting DELETE FROM QUEUE from the “Advanced actions” menu. Automatically generated special operation cards that are bound to the next process cannot be rearranged or deleted. If you wish to repeat the same sequence, choose REPEAT THIS SEQUENCE from the “Advanced actions” menu. The card listed at the very top of the queue is carried out next. The process is started by pressing the START SYNTHESIS (sequence card) or START (special operation card) button.
3 Synthesis workflow
The entire synthesis workflow consists of the automated synthesis and some user interaction steps.
3.1 Automated synthesis
The automated synthesis includes synthesis on a microfluidic chip, cleavage from the chip with automatic transfer into the PCR collection vial, deprotection at an elevated temperature and the final removal of the solvent. No user interaction is required during this time. The automated synthesis can be canceled if needed by pressing the STOP button.
CAUTION
Make sure to keep your gas supply open at all times during the synthesis process and whenever a microfluidic chip is inserted. If the gas pressure falls under a certain limit, a warning appears.
Kilobaser can stay in stand-by with inserted microfluidic chip, as long as the gas supply remains open and a sufficient input pressure is ensured. Gas supply can only be turned off when the black placeholder chip is inserted.
3.2 User interaction steps
User interaction is needed for synthesis preparation and for certain steps for starting and finishing the synthesis (described in continuation).
Inserting or changing the consumables is normally connected with motor movements to open/close the lid or to eject/insert the cartridge. The motors will never move automatically without your confirmation! The user interface clearly shows when a certain interaction is required, as well as the time until the next interaction.
Lid open
Motors start moving only after the CONFIRM button is pressed to open the lid.
CAUTION
Make sure nothing is standing on the lid.
If the free movement of the lid is obstructed in any way or if you feel that the ongoing opening will cause any kind of damage or injury, the movement can be stopped by pressing the STOP button. The motors will stop immediately and will not move until any further action is taken.
CAUTION
The lid should not be left open longer than necessary, since reagents in the cartridge are sensitive to air humidity!
Inserting a chip
After the lid has fully opened, first remove the black placeholder chip and then insert the microfluidic chip. The chip is inserted so that it is placed on the rubber nubs and fits into the chip slot, with a truncated vertex on the lower right side. Make sure to insert the appropriate type of the chip.
CAUTION
Make sure that the chip fits exactly into the slot and does not lie on the edges, otherwise the closing lid can break the chip. If you hear that the chip has cracked, stop the run immediately by pressing the STOP button.
CAUTION
Please be careful to insert a new and unused chip. Used chips should be clearly marked as they cannot be reused!
CAUTION
Because of the material properties of the rubber sealing gasket on the inside of the lid, a microfluidic chip or a placeholder can stick to its surface and stay attached to it. Make sure to remove a chip or a placeholder from the gasket! Not doing so can lead to damage of the chip or the Kilobaser. Additionally, it can lead to spillage of reagents out of the reagent cartridge.
Inserting a vial
Insert a PCR vial provided by Kilobaser into the port in the heating block. If the vial is missing, an error message will appear after the lid has closed and you will be asked to insert the vial.
Lid close
The lid starts closing after pressing the CONFIRM button.
CAUTION
Make sure there are no objects or body parts standing in a way of the closing lid, otherwise they might get squeezed! However, serious squashing injuries are prevented by the squeeze protection safety mechanism of the closing lid. If an object or a body part is only lightly squeezed by the closing lid, the obstruction is recognized and the lid starts opening again for a couple of seconds.
The movement of the closing lid can also be stopped by pressing the STOP button. The motors will stop immediately and will not move until any further action is taken.
Exchanging a cartridge
The cartridge exchange process is described in more detail later in this section.
The cartridge motor starts unlocking (moving down) the empty or expired cartridge only after you press the CONFIRM button.
CAUTION
Be careful when taking the used cartridge out of the Kilobaser, since the septa on the reagent bottles are pierced and thus not completely sealed anymore. In some cases, small amounts of liquid reagents can appear on the septa. Wear protective gloves when handling a used cartridge! Keep a used cartridge upright until disposing of it.
CAUTION
When the used cartridge is removed, do not leave the Kilobaser without a reagent cartridge longer than necessary to prevent clogging of the needles.
The cartridge motor starts locking (moving up) the new cartridge only after you press the CONFIRM button.
CAUTION
If the cartridge is not properly inserted, this is recognized by a built-in sensor, which prevents the locking of the cartridge. Inspite of the sensor, make sure to push the new reagent cartridge to the very end of the cartridge slot until you feel that it has been locked in place, otherwise Kilobaser may suffer damage beyond ordinary repair.
CAUTION
If the built-in sensor recognizes than the cartridge is removed during locking, the cartridge motor stops locking the cartridge and starts unlocking it to prevent further damage. Inspite of the sensor, do not attempt to adjust the cartridge or take it out of the cartridge slot during locking, since this might cause a serious damage to the needles of the device.
When a cartridge is moving up or down, make sure nothing obstructs its free movement. If it seems like its movement is obstructed, you can stop the motor by pressing the STOP button. Motor motion will stop immediately and remain discontinued until any further action is taken.
Choice field
When the automated synthesis is finished, the user interface shows a choice field with the following options to choose:
– Continue: Select MODIFY QUEUE if you want to add a process to the queue or modify the queue (change the sequence, change the chip type, change the order of the queued cards,…). If there is another card in the queue, its name will be shown in the left-hand part of the choice field. Select START SYNTHESIS (sequence card) or START OPERATION (special operation card) to continue. Make sure to take the vial out of the heating block, exchange the chip and insert a new vial! Follow the instructions on the screen.
– End process: If you don't want to continue, select FINISH UP in the right-hand part of the choice field. After the lid is opened, take out the vial and the used chip and insert the black placeholder chip. Make sure to insert the placeholder chip the same way as a microfluidic chip.
Before you select any of the options from the choice field, the lid remains closed. It is safe to keep Kilobaser in this state for a few hours (e.g. overnight) as long as the gas supply remains open.
CAUTION
Do not turn off the gas supply until the placeholder chip has been inserted! The cartridge and in some cases the entire device can be contaminated on a pressure drop.
After the placeholder chip has been inserted, Kilobaser can be switched off safely and the gas bottle can be turned off. The next synthesis can be started at any time. Don't forget to open the gas supply again in this case!
Removing the vial
Whether you choose to continue with the next sequence or finish up, make sure to take out the collection vial with the dried synthesized oligo. If you forget the vial in the Kilobaser, an error message will appear after the lid has closed and you will be asked to remove the vial.
CAUTION
The last steps of the automated synthesis run under an elevated temperature. When the lid is opened after a finished syntehsis, the heating block can still be hot - it will however not be hotter than 65°C (150°F). Still be careful when removing the vial from the heating block.
CAUTION
Because of the material properties of the rubber O-ring on the inside of the lid, the vial can shortly stick to its surface and get pulled out of the vial port. Please make sure to remove the vial, since the closing lid can squeeze the vial and damage it.
Synthesized oligo
The collection vial contains dried ready-to-use product. To prepare a solution, resuspend it in DNAse/RNAse free water or, preferably, a TRIS-EDTA buffer (non of the solvents is supplied by Kilobaser), vortex the solution for at least 2 minutes and then spin the vial briefly to remove bubbles and collect liquid. Usually, adding 20 μL of a solvent leads to a solution with 15 pmol/μL (15 µM; nominal yield of 300 pmol). The information about the concentration is also displayed on the sequence card and applies for the resuspension volume of 20 μL.
The exact concentration strongly depends on the sequence, length and synthesis quality (which further depends on the environmental conditions such as gas quality, air humidity and room temperature). It is recommended to validate the typical yield under your actual environmental conditions using Qubit or an equivalent fluorometric system (make sure to use a dye suitable for ssDNA).
Beside the synthesized product, the solution also contains salts from the protecting groups, unreacted reagents and small amounts of the truncated sequences. Standard DNA/RNA oligonucleotides are ready to use without further purification for standard applications such as PCR and qPCR primers. For more specific applications, validation using a positive control on the first use is recommended.
Oligonucleotide probes are ready to use for typical qPCR applications. Standard qPCR protocols may be optimized for purified probes. Unpurified Kilobaser probes contain free fluorescent dye (e.g. FAM) molecules, increasing the background signal on qPCR machines. This signal will be automatically filtered by most qPCR machines, but may require a calibration step. If in doubt, please consult the user manual of your qPCR machine. It is recommended to validate oligonucleotide probes using a positive control on the first use.
If you require standard oligonucleotides or probes desalted and with higher purity, you can use the OliPure kit.
4 Special operations
4.1 Cartridge setup
Cartridge setup is an operation that prepares Kilobaser for a new type of the reagent cartridge. It needs to be carried out in the following cases:
After storage or transportation, when the device has been without a cartridge for a while. In this case moisture can accumulate in the system and could impair the quality of the first synthesis.
After a cartridge has been contaminated and the Kilobaser has been subsequently cleaned either manually or using a cleaning cartridge.
After changing the cartridge type and subsequent cleaning. In this case small amounts of reagents (especially dyes) from the previous cartridge type can remain in the system and could have an influence on the first synthesis from the new cartridge.
When a sequence is added to the queue after the above mentioned cases, the "Cartridge setup" card is additionally automatically added. It cannot be deleted as it is bound to the next synthesis.
To only add the "Cartridge setup" card to the queue, select the appropriate cartridge type in the settings/cartridge. This card can be moved up/down the queue or removed by selecting DELETE FROM QUEUE from the “Advanced actions” menu.
For the setup process, the setup chip (included in the delivery) is used. The setup chip can be saved and used for the next cartridge setup process.
Before the new cartridge is inserted, the system is first automatically purged for 2 minutes to remove moisture. A hissing sound during this operation is normal.
The cartridge setup process is started after the new cartridge is inserted. When the setup is finished, discard the vial and continue with the next synthesis.
4.2 Open device
When Kilobaser is not synthesizing, you can open it by choosing OPEN DEVICE from the dropdown menu. The "Open device" card can be moved up/down the queue or removed by selecting DELETE FROM QUEUE from the “Advanced actions” menu.
When you open the device, keep in mind the instructions on the display and those described in the sections Lid open and Lid close of this manual.
CAUTION
Do not keep the lid open longer than necessary! Make sure to properly insert the black placeholder chip before closing the lid.
4.3 Change cartridge
After the total amount of bases in the cartridge has been used up or the cartridge has expired (by clicking on the "Consumables information" symbol, the remaining bases as well as the expiry date of the cartridge can be checked), the cartridge needs to be exchanged before a new synthesis can be started. When you add the next sequence to the queue, the additional "Change cartridge" card is automatically added. This card cannot be moved up/down the queue or deleted, since it is bound to the next synthesis. Press START to start the operation. First, the chip and vial need to be inserted: keep in mind the instructions on the display and those described in the sections Lid open, Inserting a chip, Inserting a vial and Lid close. Next, the cartridge exchange takes place: keep in mind the instructions on the display and those described in the section Exchanging a cartridge. When a new reagent cartridge is locked into the Kilobaser, an initial 20 minutes activation takes place before proceeding to the synthesis.
If you wish to exchange the cartridge before the old one has been used up or expired, you can add the "Change cartridge" card to the queue manually by choosing ADD CARTRIDGE CHANGE from the dropdown menu. This card can be moved up/down the queue or removed by selecting DELETE FROM QUEUE from the “Advanced actions” menu. Press START to start the operation. In this case, the lid remains closed and only cartridge exchange takes place. Keep in mind the instructions on the display and those described in the section Exchanging a cartridge. When the next synthesis is started, an initial 20 minutes cartridge activation takes place before proceeding to the synthesis.
CAUTION
Make sure to only start the cartridge exchange process if you have a new unused cartridge in stock.
4.4 Remove consumables
For transportation and longer storage as well as in some faulty cases, the cartridge needs to be removed from the device. In the section Remove consumables in the settings/cartridge, choose START WITHOUT CLEANING to add the "Remove consumables" card to the queue. This card can be moved up/down the queue or removed by selecting DELETE FROM QUEUE from the “Advanced actions” menu.
When you remove the cartridge, keep in mind the instructions on the display and those described in the section Exchanging a cartridge.
CAUTION
After removing the cartridge, Kilobaser needs to be cleaned manually to prevent clogging of the needles.
CAUTION
Do not perform this operation for a cartridge exchange!
4.5 Manual cleaning
After completing the “Remove consumables” operation, small amounts of reagents stay in the system and can cause internal clogging. To prevent the clogging, the system needs to be cleaned manually. The system should also be cleaned manually in rare faulty cases in which the reagents spill out of the cartridge and contaminate the system.
The process that prepares the system for a manual cleaning can be accessed in the settings/cartridge. The "Manual cleaning" card can be moved up/down the queue or removed by selecting DELETE FROM QUEUE from the “Advanced actions” menu.
If the cartridge is still in the Kilobaser, it will be moved down first. Then, to prepare the device for the manual cleaning, the lid and the cartridge motor move simultaneously. Keep in mind the instructions on the display and those described in the sections Lid open, Lid close and Exchanging a cartridge of this manual. Please make sure that in this case no cartridge is inserted!
When cleaning manually, make sure to wear personal protective equipment: safety goggles, gloves and a lab coat! The system should be cleaned with acetonitrile using a syringe (without a needle) as follows:
Put the syringe onto one rubber nub and gently press on the plunger of the syringe until acetonitrile comes out of the needle below. Make sure to put an appropriate glass container (e.g. a beaker) under the needle being cleaned to catch the acetonitrile. After washing the needle, take an empty syringe and press the air through the needle to remove the remaining acetonitrile. Repeat this procedure for all marked needles (on the display will be shown which needles must be cleaned). It is recommended to clean the needle on the top-left corner more frequently and more thoroughly.
For a detailed step-by-step manual cleaning guide download the PDF manual or watch the manual cleaning video tutorial.
4.6 Automatic cleaning
The "Automatic cleaning" card can be added to the queue by selecting START WITH CLEANING in the section Remove consumables in the settings/cartridge. This card can be moved up/down the queue or removed by selecting DELETE FROM QUEUE from the “Advanced actions” menu.
A "Change cartridge" card is additionally automatically added to the queue (which cannot be removed) to remove the used cartridge and insert the cleaning cartridge. Keep in mind the instructions on the display and those described in the sections Lid open, Lid close and Exchanging a cartridge of this manual.
Make sure to only start the process with the automatic cleaning if you have a new cleaning cartridge and a cleaning chip in stock! A cleaning cartridge can be used for only one automatic cleaning.
The cleaning process takes 10 minutes. Kilobaser can be transported or safely left standing for a longer period of time after an automatic cleaning.
Please note that it is impossible to fully remove all reagents, especially dyes from the system. It can happen that the first synthesis after the cleaning still contains small amounts of a free fluorescent dye that was previously used in the Kilobaser. A better removal of reagents can be accomplished with the manual cleaning. The cross-contamination is reduced with the cartridge setup process. To completely avoid cross-contamination, it is always recommended to use one Kilobaser with one cartridge type only.
5 Canceling operations
5.1 General cancel
Every process running on Kilobaser can be canceled at any time. To cancel the process, first press the red STOP button at the top of the display and then confirm by selecting YES. Kilobaser will automatically execute a canceling protocol. Please note that this can take up to 10 minutes. After canceling the run, the choice field appears. You can start the next opeartion from the queue, modify the queue or end the process and insert the placeholder.
If you choose to start the next synthesis, make sure to exchange the used chip and vial with new ones when the lid has opened. A chip and a vial can be reused if the synthesis is canceled in the first 30 seconds after the lid has closed.
If the STOP button is pressed accidentally and no action is taken, the process continues to run normally. The warning is either closed by selecting NO or else it automatically disappears after 5 minutes.
5.2 Lid cancel
There is a slightly different cancel mechanism for steps in which the lid is opening or closing. By pressing the STOP button, the lid stops moving immediately. This is a safety feature in case the free movement is obstructed. Make sure to remove any objects blocking the free movement of the lid before proceeding. Choosing either YES or NO from the pop-up warning will open the lid, no matter if the run was canceled on opening or closing of the lid. When the lid is fully open, the operation proceeds normally (if NO was selected from the pop-up warning) or the choice field appears (if YES was selected from the pop-up warning).
It is recommended to stop the movement of the lid only in case when something is blocking it. If nothing is obstructing the movement and you only want to cancel the current synthesis, modify the queue or in case that you do not have a new chip, it is recommended to press STOP only when the lid is fully open and is not moving anymore. Confirming the canceling with YES leads to the choice field, where you can choose the next step.
CAUTION
Please make sure that when canceling the movement of the lid, you do not leave it in a paused state for any longer than necessary, since reagents in the cartridge are sensitive to air humidity!
5.3 Cartridge cancel
If the STOP button is pressed when the cartridge is moving up/down, the cartridge stops moving immediately. This is a safety feature in case the free movement is obstructed. Please remove any objects blocking the cartridge movement. Selecting NO from the pop-up warning will continue locking/unlocking the cartridge and proceed with the process. Selecting YES will move the cartridge back to the previous position (locked or unlocked). Please use this canceling action only when free movement of the cartridge is blocked and avoid using it for cases of recklessness. Make sure to always have a new reagent or cleaning cartridge in stock and take care to insert an appropriate type of the cartridge into the cartridge slot.
If you confirm to lock the cartridge by mistake (for example wrong cartridge type), stop the motor by pressing STOP. Selecting YES will unlock the cartridge again. Insert a correct cartridge and confirm to lock it.
If you confirm to unlock the cartridge by mistake (for example if you do not have a new reagent or cleaning cartridge), stop the motor by pressing STOP. Selecting YES will lock the cartridge again. In case the used cartridge has already moved down all the way and a new cartridge is not at hand, please keep the old cartridge in the slot, press STOP and then YES to lock it again. When the cartridge has moved up all the way again, choose END PROCESS in the choice field to return to the initial state. Please only start a new synthesis with a cartridge exchange if you have an appropriate reagent cartridge in stock!