-

Print
Please Enter Your Search
search icon
Nothing found for your search
Search results from other manuals
Nothing found for your search
Version: latest

1 Step By Step Introduction

  • em Steps marked with this Symbol are crucial for the final oligonucleotide concentration. Please pay attention to this symbol and follow these steps exactly as described.

1.1 Preparation Of Magnetic Beads

  1. Magnetize the MB tube and remove storage buffer
  2. Add 100 μL dH₂O or TE buffer into one MB tube and homogenize by pipetting up and down.
  3. Place the tube in the magnetic rack.
  4. Remove supernatant while tube remains in the rack.
  5. Repeat steps 1 to 4.

1.2 Sample Addition

  1. Take the tube out of the magnetic rack.
  2. Add 100 µL BB ( red red cap) into the tube and homogenize by pipetting up and down.
  3. em Transfer max. 20 µL dissolved oligo sample directly into the liquid phase in the tube. Take care not to suck liquid from the tube back up into the pipette during this step - otherwise, you may lose oligos.
  4. em Vortex the tube intensively for 5 min. Briefly spin down any residue from the tube lid in the tabletop centrifuge.
  5. Place the tube in the magnetic rack.
  6. Remove supernatant while tube remains in the rack.

1.3 Sample Washing

  1. Take the tube out of the magnetic rack.
  2. Add 100 μL WB ( blue blue cap) into the tube slowly so that you do not disturb the pellet.
  3. Place the tube in the magnetic rack.
  4. Remove supernatant while tube remains in the rack.
  5. Repeat steps 13 to 15.
  6. Take the tube out of the magnetic rack and leave the tube open for 15 min in a closed air circulation.¹

¹ This step is only necessary to allow the acetonitrile to evaporate. Acetonitrile may interfere with some applications and long storage of oligonucleotides. For PCR applications, this step is not necessary.

1.4 Sample Elution

  1. Add 10-20 μL (depends on the desired final concentration) of dH₂0 or TE buffer into the tube and homogenize by pipetting up and down.
  2. em Vortex tube intensively for 5 min. Briefly spin down any residue from the tube lid in the tabletop centrifuge.
  3. Place the tube in the magnetic rack.
  4. Transfer the liquid phase containing the purified oligonucleotide sample into a fresh tube while the original tube remains in the rack.
  5. Discard the magnetic beads and continue with your procedure.